Sample Guidelines

Sequencing Sample Submission Guidelines

The following concentrations and volumes are recommended guidelines.

Typically, we request more volume than we need - if you need to send less, just let us know.

Sample	Concentration	Volume
PCR Product <500bp***	~40ng/µl	5-10µl
PCR Product >500bp***	~60ng/µl	5-10µl
Plasmid DNA single or 96-well*	~400ng/µl	5-10µl
Primer****	8pmol/µl	5-10µl
Bacterial Samples-Low copy	10⁹-10¹⁰ cells/ml	1-2mls
Bacterial Samples-High copy	10⁸-10⁹ cells/ml	1-2mls
Bacterial Samples-96-well**	10⁸-10⁹ cells/ml	100µl
Stab or Streak cultures	1 stab or plate	-
BAC clone	~300ng/rxn	-

*DNA should be resuspended in molecular grade water, elution buffer or as a dried down pellet. Do Not Store In TE. Lower concentration of DNA may be used in some cases.

**Bacterial Samples sent in 96-well format should be stored as glycerol stocks.

***Please do not dilute your PCR product. Typically, PCR is within the concentration range necessary for sequencing.

****We prefer the primers to be sent separately, not pre-mixed with the template. Thanks.

Shipping Guidelines

Sample Type	Container	Shipping Recommendations
PCR, Plasmid DNA or Primer (single)	0.2 -2.0 mL microcentrifuge tube	Overnight mail, blue ice or dry ice
PCR, Plasmid DNA or Primer (HT)	96-well plate	Overnight mail, frozen, dry ice
Bacterial Samples (glycerol stocks)	1.5 - 2.0 mL microcentrifuge tube, 96-well plate	Overnight mail, dry ice, frozen
Bacterial Samples (colonies/plates)	Stab or Petri Dish	Overnight mail, blue ice
BAC clones	1.5 -2.0 mL microcentrifuge tube, 96-well plate	Overnight mail, dry ice